Comparison of The Efficacy of Two Culture Media, Handmade(SOF) and Commercial (G1/G2), on In VitroDevelopment of Vitrified and Non-Vitrified Mouse Embryos

Authors
Farnoush Jafarpour
Elham Bonakdar
Mohammad Ali Edriss
Azizollah Bakhtari
Vajiheh Asgari
Sayed Morteza Hosseini
Mehdi Hajian
Mohammad Hossein Nasr-Esfahani
Abstract
There is some evidence that shows some manipulations during assisted reproductive technologies such as in vitro culture and vitrification can affect fetal development. The aim of this study was to evaluate the effect of culture medium (handmade culture medium: SOF vs. commercial culture medium: G1/G2) and vitrification (vitrified vs. non-vitrified) on survival rate, blastocyst formation and hatching rate. For this purpose, 2-cell embryos were recovered from naturally synchronized NMRI females and divided in two groups (vitrification and control groups). Embryos from each group cultured in SOF or G1/G2 culture medium. Results showed that survival rate was not significantly different between groups. The potential of embryos in vitrified-SOF group to develop towards blastocyst stage and also hatching rate were significantly lower than non-vitrified-G1/G2 group (P
Keywords
Survival rate-Blastocyst formation-Hatching rate-G?/G? and SOF culture medium-Vitrification-
Iranian Journal of Veterinary Clinical Sciences
Volume 8, Issue 2
October 2014