Evaluation of the quality of semen frozen with BoviFree extender in comparison with manual and Steridyl in dual-purpose Simmental bulls (Fleckvieh)

Document Type : Original Article

Authors
Amir Khaki 1
Shohreh Alian Samakkhah 2
Alireza Ghasemi 3
1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol-Iran
2 Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol-Iran
3 Iran Simmental Cattle Breeding Center, Amol-Iran
10.22034/ijvcs.2022.14018.1029
Abstract
The aim of this study was to investigate the quality of semen frozen with BoviFree diluent compared to manual and Steridyl in dual-purpose Simmental bulls. Semen samples were collected from 12 bulls (three replicates for each bull and a total of 36 ejaculations) using an artificial vagina and each ejaculation was divided into three equal parts. Freezing was done by two-step dilution method and sperm quality parameters such as viability, motility, morphology and plasma membrane integrity were investigated in fresh and frozen thawed semen. The results showed that all sperm characteristics of semen diluted with manual extender in fresh semen were lower than in other diluents, and this difference was significant in the case of the plasma membrane integrity. Regarding frozen thawed semen, progressive motility and other motility parameters in the group which was frozen with Steridyl diluent was significantly more than the other two groups. There was no significant difference between the group of manual and BoviFree extender. Considering the findings of our research Steridyl has a better performance in terms of sperm motility after freezing than BoviFree and manual, it can be concluded that the Steridyl diluent is more suitable for semen freezing of dual-purpose Simmental bulls than the Bovifree and manual diluent. In addition, the results of this research determined that the diluents based on egg yolk are more suitable for bull semen freezing than the diluents based on soy lecithin.

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Iranian Journal of Veterinary Clinical Sciences
Volume 17, Issue 1 - Serial Number 28
Spring & Summer 2023
September 2023
Pages 25-34