An evaluation of the effects of selenium and vitamin E supplementation on antibody titer of the Brucella melitensis strain Rev1

Brucellosis is one of the zoonotic diseases which have been considered always from economy and health point of view. The present study was done to determine that, is there any differences between the antibody of those vaccines which are injected in the early of their production to livestock, and those which are injected at the end of their production? If existing any difference, dose it has observable significant effect in the rate of produced antibodies of vaccines injected into animals by injecting a dosage of Selenium and E vitamin supplement? Animals were divided into three groups. To first group, only fresh Rev l was injected and to second group, Rev l in the final days of the shelf life was injected and to third group, Rev l in the final days of the shelf life alone with vitamin E and selenium supplementation was injected. All three groups were blood sampled on days 0, 14, 21 and 60 after vaccination. Wright technique was used to measure antibody titer in serum. None of the groups in zero day samples,had no agility of antibodies against Brucella. In all 3 groups, the overall increase in antibody production was observed to days 60. Despite higher antibody titer in animals that received supplementation; there was no statistically significant difference in different Groups. single dose of vitamin E and selenium, simultaneously with injecting vaccines, it couldn't result in significant increase in antibody mass in sheep.