sperm treatment with effective compounds on sperm chromatin in intracytoplasmic sperm injection (ICSI) procedure

Authors
arefeh golestanfar
Abolfazl shirazi (Corresponding author)
naser Shams Esfandabadi
Ebrahim ahmadi
Abstract
Considering the low percentages of sperm chromatin decondensation and male pronuclear formation following intracytoplasmic sperm injection (ICSI) in ovine species which decrease the chance of normal fertilization, the current study was designed to assess the effects of some methods of sperm pretreatment on male pronuclear (MPN) formation and oocyte activation in ovine ICSI procedure.
The experimental groups of this study were comprised of: I) ICSI of untreated sperm (control), II) ICSI of pretreated sperm with GSH+Hep (GSH+Hep), III) ICSI of pretreated sperm with GSH+Hep coinjected with GSH (GSH+Hep+inj.GSH), and IV) ICSI of pretreated sperm with GSH+Hep coinjected with sperm extract (GSH+Hep+inj.SE). The injected oocytes were activated with Io+6-DAMP and were examined for prenuclear formation after 16h. The MPN formation in GSH+Hep+inj.GSH was higher (P
Keywords
glutathione.-oocyte activation-Key words: sperm decondensation-ICSI-