Evaluating the effects of different concentrations of glycerol and thawing temperatures in the process of ram epididymal spermatozoa cryopreservation

Authors
Ebrahim Ahmadi
Hassan Nazari
najmeh davoodian
Abstract
Cryopreservation of epididymal spermatozoa has an important role in the conservation programs of endangered domestic and wild animal species, as well as, maintaining the genetic potential of suddenly dead valuable males. The aim of the present study was setting up an optimum protocol for the cryopreservation of ram epididymal spermatozoa. Spermatozoa extracted from the cauda epididymis of slaugtherd rams were frozen in the extenders containing 2, 4 and 6 percent glycerol and were thawed at 37 and 65?C. Post-thawing motility parameters were evaluated by CASA. The factorial ANOVA model were used to determine the statistical differences between the main effects of the glycerol concentrations, thawing temperatures and their interactions (concentration×temperature). Because a non-significant interaction was detected, the main effects (concentration and temperature) were interpreted. The effect of glycerol concentration on the all motility parameters and the effect of thawing temperature on the majority of motility parameters were significant (P
Keywords
Epididymal spermatozoa
Glycerol concentration
Thawing temperature
Cryopreservation