Improving the growth of bovine isolated ovarian secondary follicles with Akt signaling pathway stimulation

Authors
ُSima Hemmatian Khayyat
abolfazl Shirazi
Naser Shams Esfandabadi
Seyed Abbas Mirzaie
Hassan Nazari
Abstract
In vitro ovarian follicle culture in females at the risk of losing fertility, such premature ovarian failure (POF) and in those subjected to chemo/radio therapy as well as in animals – species at risk of extinction in a promising strategy for fertility preservation. Development of ovarian growing follicles is regulated by the coordinated action of several signaling pathways. The use of some activators or inhibitors of signaling pathways is effective in optimizing the culture conditions of immature follicles. The aim of this study was examining in vitro growth of bovine ovarian secondary stage follicles (> ?? ?m) by mimicking the process of in vivo follicular development through of the Akt signaling pathway activator, PS??. Small secondary stage follicles were isolated by enzymatic digestion from slaughter ovaries by collagenase type I and Dnase I, cultured in the presence of BSA (? mg/ml), FBS (??%), Akt (?µM PS??, first ?? h of culture) or Akt + FBS (?µM PS??, first ?? h of culture + ??% FBS) for ten days and then evaluated for growth rate. The follicular size was significantly increased in all experimental groups during ?? days culture (P
Keywords
Cattle
In vitro culture
Secondary Follicles
Akt signaling pathway
PS??