Distribution, morpholy and Phylogenetic Study of Hypoderma bovis by PCR and CO1 gene sequence analysisin in three climate regions in Iran

Hypoderma bovis (Diptera: Oestridae) is an important parasite in cattle that this parasite is pathogenic and cause serious economic losses that result to reduce livestock products, including milk, meat and hide quality. In this study, samples from slaughtered cattle were collected from three climate of Iran. Extraction of DNA and PCR test was performed on samples, then the purified PCR product was sent for sequencing. Sequence response was reviewed and corrected. Finally, the distribution of this species was investigated according to morphological characteristics in three climate zones of Iran. CO? gene of third stage larvae of H. bovis was investigated in collected larvae. Data showed interspecies correlation. Based on morphological observations, from total of ??? third stage larvae obtained from slaughtered cattle in three regions, ??? (??.?%) were H.bovis. Based on phylogenetic analysis of the present isolates, these isolates are located in a cluster with a number of Hypoderma species of studied in other countries, while some other species are isolated in the other cluster. The COl region seems to be very variable among the miyasis caused flies. The findings also suggest that CO? gene can be used as an appropriate molecular marker for the identification and diagnosis of Hypoderma species.