Evaluation of an in-house enzyme linked immuonosorbent assay (ELISA) for detection of anti-Oestrus ovis antibodies in sheep

Oestrus ovis is a fly with cosmopolitan dispersion and one of zoonotic agents. The larvae are a common cause of myiasis in human and sheep. This study was conducted in first time in Iran to evaluate an ELISA for diagnosis of Oestrus ovis infection in sheep by using somatic (S) and excretory-secretory (ES) antigens from the second and third stage larvae of the parasite (L?, L?) collected of the study area. Somatic/excretory-secretory antigens were prepared by homogenization and culturing the larvae in RRMI-???? respectively. After determination of appropriate dilutions of the antigens, serum, conjugate. evaluation of ELISA for diagnosis of anti-Oestrus ovis antibodies in infected sheep were examined with the antigens, ?? positive and ?? negative (from indoor lambs) serum samples. The results of this study revealed appropriate dilutions of the SL? and SL? ?:??, ?:??, conjugate (?:????) and sera (?:??) and also of ESL? and ESL?, ?:?, ?:?, conjugate (?:????) and sera (?:?) respectively. The sensitivity and specificity rates of the assay with the SL? and SL? were ??.?%, ??.?%; ??.?%, ??.?% and with ESL? and ESL?, ??.?%, ??.?%; and ??.?%, ??.?% respectively. Positive / negative predictive values of the test with the SL? and SL? were ??.?%, ??.?% and ??.?%, ??.?% while with the ESL? and ESL? were ??.?%, ??.?% and ??.?%, ??.?% respectively. According to the results, L? was better than L?, and somatic antigens compared with excretory-secretory antigens revealed better results, thus it can be used for serodiagnosis and epidemiological studies of oestrosis in sheep.