Improving Quality of Vitrified-Warmed Ovine Immature Oocytes by Using Glycine

Authors
Ebrahim Ahmadi
Aboalfazl Shirazi
Naser Shams-Esfandababi
Hassan Nazari
Abstract
Despite the successful pregnancies and live births that have been reported from transferring embryos derived from cryopreserved oocytes in several mammalian species, developmental competence of frozen-thawed oocytes is low. The aim of this study was examining the effects of short term treating of immature ovine oocyte with glycine and supplementation of vitrification and warming solution with glycine on post vitrification and warming quality. Abattoir-derived immature ovine oocytes vitrified with (GLY) or without (CON) treating with glycine and after In vitro maturation the nuclear maturation and the rate of apoptosis of them compared with their unvitrified (FRESH) counterparts. A difference at a level of P

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