Experimental vertical transmission of different doses of Salmonella Enteritids in Broilers chickens

Salmonellosis is one of the most important food-borne diseases. Outbreaks of Salmonellosis are related to the consumption of contaminated eggs, egg-products or poultry meat. Salmonella has the unique ability to contaminate eggs without causing discernible illness in the birds infected. In this study, two groups of fertile eggs were inoculated with 170 (A) and 17 (B) CFU of parent strains of S. Enteritidis (S.E). Contaminated eggs were incubated in different setters and survived chickens were hatched. At days 2, 7, 14, 21 and 28 of rearing period, samples were taken from cloaca and different parts of digestive system of euthanized birds. High rate of Salmonella contamination were observed in cloacal swabs of group A eggs; Salmonella contamination of caecum, intestines and livers of group A chickens in early and late days of experimental period were more higher than group B chickens. Using PCR test, four genes for S. Enteritidis identification were detected from isolated colonies of two group eggs and chickens.