Evaluation the prevalence of persistent post-mating endometritis in Arabian mares

Document Type : Original Article

Authors
Yaser Nazem 1
Naser Shams Esfandabadi 1
Ali Kadivar 2
Najmeh Davoodian 3
Hasan Nazari 3
1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord- Iran
2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord- Iran.
3 Research institute of Animal Embryo Technology, Shahrekord University, Shahrekord- Iran
10.22034/ijvcs.2023.14297.1042
Abstract
The incidence of persistent post-mating endometritis (PPME) following natural mating or artificial insemination (AI) is always underestimated in mares that do not have abnormal clinical signs in reproductive tracts in routine examinations before mating in high-population horse breeding centers. In this study, 503 Arabian mares whose reproductive system status was confirmed before natural mating or artificial insemination in breeding soundness examination, were examined with ultrasonography at 24, 48, and 72 hours after ovulation, in terms of PPME following natural mating or AI, and the presence of uterine fluid accumulation with a diameter of 5 mm or more is recorded as PPME. The correlation between PPME and other variables such as age, type of mating, number of mattings in an estrous cycle, pre-ovulatory follicle (POF) size, volume and concentration of semen used in AI was investigated at different time intervals after ovulation. The results of this study showed that, the incidence rate of PPME following natural mating or AI in 48 and 72 hours after ovulation is 32 and 22%, respectively, in a normal population of Arab mares. Based on this rate, reproductive examinations are recommended 24 and 48 hours after ovulation in the group of sensitive Arab mares.
Keywords
Mare
Post mating
Persistent endometritis
Prevalence
Arab horse
Ultrasonography
Subjects
 1- Abo-El maaty, A.M. and El-Shahat, K.H; Hormonal and biochemical serum assay in relation to the estrous cycle and follicular growth in Arabian mare. Asian Pac.J.Reprod; 2012; 1:105-110.
2- Alghamdi, A.S; Foster, D.N; Carlson, C.S. and Troedsson, M.H.T; Nitric oxide levels and nitric oxide synthase expression in uterine samples from mares susceptible and resistant to persistent breeding-induced endometritis. Am.J.Reprod.Immunol; 2005; 53:230-237.
3- Allen, W.R; Brown, L; Wright, M. and Wilsher, S; Reproductive efficiency of Flatrace and National Hunt Thoroughbred mares and stallions in England. Equine Vet. J; 2007; 39:438-445.
4- Auclair-Ronzaud, J; Jaffrézic, F; Wimel, L; Dubois, C; Laloë, D. and Chavatte-Palmer, P; Estimation of milk production in suckling mares and factors influencing their milk yield. Animal; 2022; 16:100498.‏
5- Canisso, I.F. Stewart, J. Coutinho da Silva, M.A. Endometritis: Managing persistent post-breeding endometritis.Vet. Clin. N. Am. Equine.Pract. 2016; 32, 465–480.
6- Canisso, I.F; Segabinazzi,L.G. and Fedorka, C.E; Persistent breeding-induced endometritis in mares—A multifaceted challenge: From clinical aspects to immunopathogenesis and pathobiology. Int.J.Mol.Sci; 2020; 21:1432.
7- Carnevale, E.M; Ramirez, R.J; Squires, E.L; Alvarenga, M.A; Vanderwall, D.K. and McCue, P.M; Factors affecting pregnancy rates and early embryonic death after equine embryo transfer. Theriogenology; 2000; 54:965–979.
8- Causey, R.C; Miletello, T; O’Donnell, L; Lyle, S.K; Paccamonti, D.L; Anderson, K.J; Eilts, B.E; Morse, S. and LeBlanc, M.M; Pathologic Effects of Clinical uterine inflammation on the equine endometrial mucosa. American Association of Equine Practitioners; 2008; 54:276–277.
9- Evans, M.J; Hamer, J.M. Gason, L.M. and Irvine A.C; Factors affecting
uterine clearance of inoculated materials in mares. J.Reprod.Fert; 1987; 35:327-342.
10- Evans, M.J; Hamer, J.M; Gason, L.M; Graham, C.S; Asbury, A.C. and Irvine C.H; Clearance of bacteria and non-antigenic markers following intra-uterine inoculation into maiden mares: Effect of steroid hormone environment.  Theriogenology; 1986; 26:37–50
11- Fiala, S.M; Pimentel, C.A; Mattos, A. L.G; Gregory R.M. and Mattos R.C; Effect of sperm numbers and concentration on sperm transport and uterine inflammatory response in the mare. Theriogenology; 2007; 67:556-562.
12- Hurtgen, J.P; Pathogenesis and treatment of endometritis in the mare: A review. Theriogenology; 2006; 66:560–566.
13- Kotilainen, T; Huhtinen, M. and Katila, T; Sperm-induced leukocytosis in the equine uterus. Theriogenology; 1994; 41:629-636.
14- LeBlanc, M.M; Magsig, J. and Stromberg, A.J; Use of a low volume uterine flush for diagnosing endometritis in chronically infertile mares. Theriogenology; 2007; 68:403-412.
15- LeBlanc, M; Advances in the diagnosis and treatment of chronic infectious and post-mating-induced endometritis in the mare. Reprod.Domest.Anim; 2010; 45:21–27.
16- LeBlanc, M. and Causey, R; Clinical and subclinical endometritis in the mare: both threats to fertility. Reprod.Domest.Anim; 2009; 44:10–22.
17- Maischberger, E ; Irwin, J.A; Carrington, S.D. and Duggan, V.E; Equine post-breeding endometritis: A review. Ir.Vet.J; 2008; 61:163-168.
18- Morel, M.C.D; Newcombe, J.R. and Swindlehurst J.C. The effect of age on multiple ovulation rates, multiple pregnancy rates and embryonic vesicle diameter in the mare. Theriogenology; 2005; 63: 2482-2493.
19- Neely, D.P; Kindahl, H; Stabenfeldt, G.H; Edqvist, L.E. and Hughes, J.P; Prostaglandin release patterns in the mare: physiological, pathophysiological, and therapeutic responses. J. Reprod. Fertil; 1979; 27:181–189.
20- Pycock, J.F. and Allen, W.E; Inflammatory components in uterine fluid from mares with experimentally induced bacterial endometritis. Equine.Vet.J; 1990; 22:422-425.
21- Pycock, J.F; Breeding management of the problem mare. Equine Breeding Management and Artificial Insemination. 2th.Ed. Saunders Elsevier, St Louis, CA, 2009; pp:139-164.
22- Scoggin, C.F; Not just a number: effect of age on fertility, pregnancy and offspring vigour in Thoroughbred brood-mares. Reprod. Fertil. Dev; 2015; 27:872-879.
23- Traub-Dargatz, J.L; Salman, M.D and Voss, J.L; Medical problems of adult horses, as ranked by equine practitioners. J. Am. Vet. Med. Assoc; 1991; 198: 1745-1747.
24- Troedsson, M.H.T; Uterine response to semen deposition in the mare. Proc. Soc. Theriogenol; 1995; 130-135.
25- Troedsson, M.H; Liu, I.K. and Crabo, B; Sperm transport and survival in the mare. Theriogenology; 1998; 49:905-915.
26- Troedsson, M.H.T; Therapeutic considerations for mating-induced endometritis. Pferdeheilkunde 1997; 13: 516-520.
27-  Troedsson, M.H.T; Uterine clearance and resistance to persistent endometritis in the mare. Theriogenology; 1999; 52: 461-471.
28-  Warriach H. M; Memon, M. A;  Ahmad, N;  Norman, S. T; Ghafar, A. and  Arif M; Reproductive performance of arabian and Thoroughbred mares under subtropical conditions of Pakistan. Asian-Australas.J.Anim.Sci; 2014; 27: 932-936.
29- Zent, W.A; Troedsson, M.H.T. and Xue J.L; Postbreeding uterine fluid accumulation in a normal population of Thoroughbred mares: a field study. Proc. Am. Assoc. Equine Pract; 1998; 44: 64-65.
Iranian Journal of Veterinary Clinical Sciences
Volume 17, Issue 1 - Serial Number 28
Spring & Summer 2023
September 2023
Pages 71-79