The effects of 1,8cineole and Ibuprofen on Glutathione Reductase in rats with hyperammonemia

Investigation of ibuprofen (Ibu) alone and in combination with 1,8-cineol (Cin) effects on the anxiety behavior and oxidative stress in male rats was aimed in this study. A total of 36 rats were divided into six equal groups including the control (recived normal saline), Ibu, Cin5, Cin 10 and Ibu+Cin. Intra-peritoneal injection of ammonium acetate for 4 weeks was used to induce hyperammonia. The animal were then adminstated with normal saline, 15 mg Ibu, 5 and 10 mg Cin and 10 mg Cin +15 mg Ibu/Kg, respectively. Morris water maze and Elevated plus maze was used respectively for evaluation of spatial memory and evaluation of anxiety. Glutathione reductase was measured for evaluation of oxidative stress. The expression levels of IL-6 and IL-1β were evaluated using Real-time PCR in brain tissue.The results showed a significant increase in spatial memory and a significant decrease in axiety behavior (p<0.01) in Ibu group compared to the control group, but no significant change(p>0.05) was observed in glutathione reductase levels. In addition to significant increase in spatial memory and decrease in anxiety behavior in Ibu-Cin group, a significant increase in glutathione reductase levels was seen(p<0.01).The expression level of IL-6 and IL-1β in Ibu-Cin5 and 10, and Ibu+Cin were significantly down-regulated compared to the control group.The results showed that the combination of Ibu and Cin reduces oxidative stress in addition to spatial memory enhancement and reduction of anxiety behavior; therefore, simultaneous application of these two compounds may be beneficial in improvement of hyperammonia-induced anxiety and cognitive impairments.