The Effect of Homologous Platelet Rich Plasma on Histopathology of Testicular Tissue in Rats Affected by Acute Toxemia Induced by Cadmium Chloride

Reducing infertility is becoming a growing issue worldwide.  The main cause of male infertility is the lack of sperm function and ultimately leads to the loss of fertility. One of the main reasons for the loss of function is related to intercellular oxidative stress. Oxidative stress is a common factor in about half of the infertile men and has been investigated. Although testicular tissue expresses antioxidant enzymes such as superoxide dismutase, catalase and glutathione peroxidase to cope with oxidative stress, but their levels are greatly reduced by cadmium exposure, which indicates the importance of cadmium as an inducer of oxidative stress. Cadmium disrupts the reproductive capacity by causing severe destruction of the testis, seminiferous tubule damage and necrosis in rat, and following acute exposure to this poisoning, the damage caused by cadmium can be found in the interstitial and tubular levels. In this study, 30 adult male Wistar rats weighing 150-200 grams were studied in three groups: control group (Normal saline as an intraperitoneal injection), cadmium group (Intraperitoneal injection of cadmium chloride with a dose of 1.2 mg/Kg), and treatment group (Intraperitoneal injection of cadmium chloride with a dose of 1.2 mg/Kg and intratesticular injection of PRP). Sampling was done on the 30th and 60th day after the start of the study. The pathology results of this study show that in the control group, there is no microscopic damage, and in the cadmium group, the testicular tissue is extensively destroyed and lacks any functional ability.  In the treatment group, severe and extensive necrosis was observed, and the remaining tubes were without spermatozoa, and calcification was forming in some parts of the seminiferous tubules.  The results of the present study show that PRP treatment cannot effectively overcome the effects of acute cadmium poisoning.