Effect of Vitamin E and Coenzyme Q10 on Experimental Fracture in Japanese Quail (Coturnix japonica): Histopathological Evaluation

Document Type : Original Article

Authors
Moosa Javdani 1
Shirin Mahdian 1
Seyyed Sattar Tohidifar 1
Mohammad Hashemnia 2
1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord- Iran.
2 Department of Basic Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah- Iran.
10.22034/ijvcs.2025.15246.1140
Abstract
This study investigated the effects of vitamin E and coenzyme Q10 on the healing process of experimentally induced fractures in Japanese quail. 40 adult male quails were randomly divided into four groups: Control, Q10 (30 mg/kg feed), Vitamin E (100 mg/kg feed), and a combination of vitamin E and Q10. Fractures were surgically created and stabilized using internal pins. Tissue samples were collected at days 7, 14, and 21 post-surgery for histopathological examination. At day 7, control birds exhibited primarily fibrous tissue and newly formed blood vessels, while treated groups showed a significant increase in fibroblasts and vascularization. By day 14, control samples consisted mainly of fibrous tissue with minimal hyaline cartilage, whereas the treatment groups displayed predominantly hyaline cartilage in the callus. At day 21, control animals mainly had fibrous and vascular tissue, whereas the groups receiving treatments showed small islands of hyaline cartilage, fibrous tissue, and newly formed bone, indicating advanced healing. Notably, the combination of vitamin E and Q10 produced the highest level of endochondral ossification and overall fracture repair. The results suggest that the synergistic effects of vitamin E and Q10 accelerate and enhance bone regeneration in avian models, with the combined therapy being most effective. These findings highlight the potential benefits of antioxidant supplementation in improving fracture healing.

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Iranian Journal of Veterinary Clinical Sciences
Volume 19, Issue 1 - Serial Number 32
Spring & Summer 2025
August 2025
Pages 85-93