Evaluation of the effect of two colibacillosis induction methods and treatment with florfenicol on selected serum biochemical parameters in broiler chickens

This study compares the effect of two colibacillosis induction methods including intratracheal and subcutaneous routes and treatment with florfenicol on serum parameters of broilers. One hundred and twenty five 35-day-old broilers were divided into 3 control groups (healthy, intratracheal induction control, subcutaneous induction control) and 4 experimental groups (intratracheal induction, intratracheal induction and antibiotic treated, subcutaneous induction, subcutaneous induction and antibiotic treated). Three days after treatment, blood sampling from all groups was performed. CPK activity in the four experimental groups showed a significant increase compared to the control groups. Uric acid in two groups of subcutaneous induction and subcutaneous induction with antibiotic treatment had a significant increase compared to the control groups. Sodium showed a significant increase in the intratracheal induction with antibiotic treatment group compared to the control groups. Potassium, phosphorus, AST, LDH and GGT in the treatment groups did not change significantly compared to the control groups (p<0.05). Taken together, kidney damage in birds inoculated by subcutaneous method is higher than intratracheal method according to the level of uric acid as a serum parameter of kidney function. Both challenge methods do not result in liver damage according to liver parameters, while a strong increase in CPK activity is present in both challenge methods. Florfenicol does not significantly change the serum parameters of birds with colibacillosis (except the amount of sodium in the intratracheal method).