تشخیص مولکولی تریکوموناس گالینه با استفاده از تکثیر ژن rRNA 5.8S به روش PCR در کبوتران خانواده کلمبیده (Columbidae)

Trichomonas gallinae is ?agellated protozoan that caused avian trichomonosis. This organism is widespread in clombiforms. In most cases infection with T.gallinae in birds can be asymptomatic. Currently, diagnosis of the organism is by laboratory assays included wet spread, in vitro culture and molecular methods.
In this study, using by Polymerase Chain Reaction( PCR) method and based on 5.8S rRNA gene amplification T.gallinae examined and the results compared with wet test. This study carried out for investigation of trichomonase contamination of 72 pigeon of clombidae family that included 2 Laughing Dove(Spilopelia senegalensis), 8 Rock Dove(Columba livia) and 62 Domestic Pigeon(Columba livia domestica) . In this examination the samples were gathered from mouth and larynx of those pigeons by soap and then each sample was divided into two parts. Immediately a part of it was inspected via wet spread slide method that 18 positive samples were detected with this method. Another part of sample was used for specific PCR reaction using a primer pair of 5.8S ribosomal gene protected region was used T.gallinae with this method 24 positive samples were detected.
The molecular results showed that PCR with primers Trich-1Fand Trich-1R yielded 390–base pair amplicon. As well as it can be saied the PCR test is higher sensitivity and specificity than wet test.