The effect of Levamisole on the immune response and histopathological conversions in high density of rainbow trout (Oncorrhynchus mykiss)

Authors
Saeid Meshkini
Naser Agh
Ali Asghar Tehrani
Abstract
In this investigation we evaluated the effects of Levamisole on histopathological changes and immune responses of rainbow trout. 1500 fish (average weight 150 g) were obtained from a local fish farm of Urmia and were divided in 5 treatments and were fed on diet supplemented with Levamisole at 0 (control), 100, 250, 500 and 1000 mg kg-1 diet for a period of 45 days. Then the fishes of all groups were fed on commercial diet without Levamisole and were exposed density stress by 2 folds (150 kg/m3) for the following 15 days. Blood samples were collected from all treatments on days 15, 30, 45 and 60 to evaluate the compliment activity and lysozyme activity of serum; and results were analyzed with SPSS15 software. On the end of trial, tissue samples of gill, kidney and liver were also collected to test the histopathologic effects of Levamisole. At the end of trial period, results showed increase levels of lysozyme activity and compliment activity (P
Keywords
Levamisole-Immune responses-Histopathological changes-Rainbow trout-