بررسی اثرات نانوزئولیت در روش تزریق داخل تخم‌مرغی بر سمیت رویان و ناهنجاری‌های مادرزادی جوجه مرغ: نگرشی به آسیب‌شناسی بافتی و ایمونوهیستوشیمی

نوع مقاله : مقاله پژوهشی

نویسندگان
محمد رسول امینی 1
مرضیه حجازی 2
1 1- دانشجوی دکتری، گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد. ایران
2 استادیار، گروه علوم پایه، دانشکده دامپزشکی، دانشگاه تبریز، تبریز. ایران
10.22034/ijvcs.2024.14429.1050
چکیده
زئولیت‌ها ساختار‌های کریستالی آلومینوسیلیکات هستند که ساختار متخلخل داشته و قادر به جذب و آزادسازی مواد هستند. در دامپزشکی از زئولیت‌ها به عنوان افزودنی غذای حیوانات، تصفیه آمونیاک استخر ماهی، کنترل بو و رطوبت حیوانات خانگی، جذب مایکوتوکسین‌ها و کاهش پاتوژن‌های روده استفاده می‌گردد. این تحقیق به منظور بررسی ضایعات هیستوپاتولوژیک احتمالی زئولیت‌ها در کبد و کلیه جوجه مرغ طراحی شده است. 80 عدد تخم مرغ نطفه دار از مرغ نژاد رأس ۳۰۸ تهیه و به طور تصادفی به ۴ گروه ۲۰ تایی (یک گروه کنترل و سه گروه آزمایش) تقسیم شدند. در گروه کنترل به میزان ۳/۰ میلی لیتر سرم فیزیولوژی تزریق و در گروه‌های آزمایش به ترتیب ۳/۰ میلی لیتر از محلول نانوزئولیت (۵، ۱۰ و ۱۰۰ میلی گرم بر لیتر) به کیسه هوایی تخم مرغ تزریق شد. سپس تخم مرغ‌ها در دستگاه هچری قرار داده شدتد و در پایان روز ۲۰ انکوباسیون، از جنین‌ها نمونه کبد و کلیه گرفته شد. نتایج هیستوپاتولوژیک کلیه، گروه دریافت‌کننده نانوذره زئولیت با دوز ۱۰۰ میلی گرم بر کیلوگرم از نظر علایم پاتولوژیک با بقیه گروه ها اختلاف معنی‌دار داشت (05/0>P). نشان داده شد که غلظت‌های مختلف نانوذرات زئولیت بر روی بافت کبد جنین اثر گذاشته و تغییرات پاتولوژیک بافت کبد با افزایش دوز بیشتر شد (05/0>P). در بررسی ایمنوهیستوشیمی میان گروه‌های تیمار با نانوذرات زئولیت و گروه کنترل اختلاف معنی‌دار مشاهده نشد (05/0
کلیدواژه‌ها
نانوزئولیت
جنین جوجه
ناهنجاری‌های مادرزادی
آسیب‌شناسی بافتی
ایمونوهیستوشیمی
موضوعات

عنوان مقاله English

Investigation of the effects of nanozeolite in the in ovo injection method on the toxicity of the embryo and congenital abnormalities in chicks: A perspective on Histopathology and immunohistochemistry

نویسندگان English

mohammad rasoul amini 1
marzieh hejazi 2
1 1- Department of Avian Diseases, Faculty of Veterinary Medicine, University of Shahre kord, Shahre kord, Iran
2 Assistant Professor, Basic Science Department, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
چکیده English

Zeolites are crystal structures that have a porous structure and are able to absorb and release substances. In veterinary medicine, zeolites are used as animal feed additives, fishpond ammonia purification, pet odor and moisture control, absorption of mycotoxins and reduction of intestinal pathogens. This research was designed to investigate possible histopathological lesions in the liver and kidney of chickens. 80 fertilized eggs were prepared from Ras 308 chickens and randomly divided into 4 groups of 20 (one control group and three experimental groups). In the control group, 0.3 ml of physiological serum was injected, and in the experimental groups, 0.3 ml of nanozeolite solution (5, 10 and 100 mg/liter) was injected into the air sac of the egg. Then eggs were placed in the hatchery machine and at the end of the 20th day of incubation, liver and kidney samples were taken from the embryos. The histopathological results of the kidney, the group receiving zeolite nanoparticles with a dose of 100 mg/kg had a significant difference with the rest of the groups in terms of pathological symptoms (P<0.05). It was shown that different concentrations of zeolite nanoparticles had an effect on fetal liver tissue and the pathological changes of liver tissue increased with increasing dose (P<0.05). In the immunohistochemistry analysis, no significant difference was observed between the treatment groups with zeolite nanoparticles and the control group (P>0.05). The results of this study showed that excessive use of zeolite nanoparticles in various industries may have long-term harmful effects on living organisms.

کلیدواژه‌ها English

nanozeolite
chick embryo
teratogenic
histopathology
immunohistochemistry
1.     Ahamed M, A Alhadlaq H, Alam J, Khan M, Ali D, Alarafi S. Iron oxide nanoparticle-induced oxidative stress and genotoxicity in human skin epithelial and lung epithelial cell lines. Current pharmaceutical design. 2013; 19(37): 6681-90.
2.     Ahamed M, Ali D, Alhadlaq HA, Akhtar MJ. Nickel oxide nanoparticles exert cytotoxicity via oxidative stress and induce apoptotic response in human liver cells (HepG2). Chemosphere. 2013 (b); 93(10): 2514-22.
3.     Ali N. Teratology in zebrafish embryos: a tool for risk assessment (Doctoral dissertation, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences). 2007.
4.     Baratli Y, Charles AL, Wolff V, Ben Tahar L, Smiri L, Bouitbir J, Zoll J, Sakly M, Auger C, Vogel T, Abdelmelek H. Age modulates Fe 3 O 4 nanoparticles liver toxicity: dose-dependent decrease in mitochondrial respiratory chain complexes activities and coupling in middle-aged as compared to young rats. BioMed Research International. 2014; 2014.
5.     Barlow S, Chesson A, Collins JD, Flynn A, Hardy A, Jany KD, Knaap A, Kuiper H, Larsen JC, Le Neindre P, Schans J. The potential risks arising from nanoscience and nanotechnologies on food and feed safety. EFSA JOURNAL. 2009;7(3).
6.     Bishop JB, Witt KL, Sloane RA. Genetic toxicities of human teratogens. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 1997; 396(1-2): 9-43.
7.     Buzea C, Pacheco II, Robbie K. Nanomaterials and nanoparticles: sources and toxicity. Biointerphases. 2007 Dec 1;2(4):MR17-71.
8.     D’arcy MS. Cell death: a review of the major forms of apoptosis, necrosis and autophagy. Cell biology international. 2019 Jun;43(6):582-92.
9.     European Food Safety Authority (EFSA), Schoonjans R. Annual report of the EFSA Scientific Network of Risk Assessment of Nanotechnologies in Food and Feed for 2016. 2016 Dec.
10.  Grodzik M, Sawosz E. The influence of silver nanoparticles on chick embryo development and bursa of Fabricius morphology. Journal of Animal and Feed Sciences. 2006; 15: 111.
11.  Hassanvand A, Hajihassani M, Abdi M, Gharibzadeh S. Drug delivery using nano-pore zeolites and ultrasound. The Journal of Neuropsychiatry and Clinical Neurosciences. 2013; 25(1): E20-.
12.  Hejazy M, Moradi M, Akbari G, Amini MR. Investigation on the teratogenic and embryotoxic effects of nanozeolite on chick embryos model. Nanomedicine Research Journal. 2018; 3(3): 169-73.
13.  Jamkhande PG, Chintawar KD, Chandak PG. Teratogenicity: a mechanism based short review on common teratogenic agents. Asian Pacific Journal of Tropical Disease. 2014; 4(6): 421-32.
14.  Karamanlis X, Fortomaris P, Arsenos G, Dosis I, Papaioannou D, Batzios C, Kamarianos A. The effect of a natural zeolite (clinoptilolite) on the performance of broiler chickens and the quality of their litter. Asian-Australasian Journal of Animal Sciences. 2008; 21(11): 1642-50.
15.  Kihara T, Zhang Y, Hu Y, Mao Q, Tang Y, Miyake J. Effect of composition, morphology and size of nanozeolite on its in vitro cytotoxicity. Journal of bioscience and bioengineering. 2011 Jun 1;111(6):725-30.
16.  Kohen R, Nyska A. Invited review: oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicologic pathology. 2002; 30(6): 620-50.
17.  Kyrylkova K, Kyryachenko S, Leid M, Kioussi C. Detection of apoptosis by TUNEL assay. Odontogenesis: Methods and Protocols. 2012: 41-7.
18.  Laban G, Nies LF, Turco RF, Bickham JW, Sepúlveda MS. The effects of silver nanoparticles on fathead minnow (Pimephales promelas) embryos. Ecotoxicology. 2010; 19: 185-95.
19.  Lee KJ, Browning LM, Nallathamby PD, Xu XH. Study of charge-dependent transport and toxicity of peptide-functionalized silver nanoparticles using zebrafish embryos and single nanoparticle plasmonic spectroscopy. Chemical research in toxicology. 2013; 26(6): 904-17.
20.  Mazzu-Nascimento T, Melo DG, Morbioli GG, Carrilho E, Vianna FS, Silva AA, Schuler-Faccini L. Teratogens: a public health issue–a Brazilian overview. Genetics and Molecular Biology. 2017; 40: 387-97.
21.  Męczyńska-Wielgosz S, Piotrowska A, Majkowska-Pilip A, Bilewicz A, Kruszewski M. Effect of surface functionalization on the cellular uptake and toxicity of nanozeolite A. Nanoscale research letters. 2016; 11: 1-4.
22.  Mellaerts R, Delvaux J, Levêque P, Wuyts B, Van den Mooter G, Augustijns P, Gallez B, Hermans I, Martens J. Screening protocol for identifying inorganic oxides with anti-oxidant and pro-oxidant activity for biomedical, environmental and food preservation applications. Rsc Advances. 2013; 3(3): 900-9.
23.  Park MR, Gurunathan S, Choi YJ, Kwon DN, Han JW, Cho SG, Park C, Seo HG, Kim JH. Chitosan nanoparticles cause pre-and postimplantation embryo complications in mice. Biology of reproduction. 2013; 88(4): 88-1.
24.  Rashidi H, Sottile V. The chick embryo: hatching a model for contemporary biomedical research. Bioessays. 2009; 31(4): 459-65.
25.  Rogers NJ, Franklin NM, Apte SC, Batley GE. The importance of physical and chemical characterization in nanoparticle toxicity studies. Integrated Environmental Assessment and Management: An International Journal. 2007; 3(2): 303-4.
26.  Roman D, Yasmeen A, Mireuta M, Stiharu I, Al Moustafa AE. Significant toxic role for single-walled carbon nanotubes during normal embryogenesis. Nanomedicine: Nanotechnology, Biology and Medicine. 2013; 9(7): 945-50.
27.  Salvestrini S, Sagliano P, Iovino P, Capasso S, Colella C. Atrazine adsorption by acid-activated zeolite-rich tuffs. Applied Clay Science. 2010; 49(3): 330-5.
28.  Swain P, Nayak SK, Sasmal A, Behera T, Barik SK, Swain SK, Mishra SS, Sen AK, Das JK, Jayasankar P. Antimicrobial activity of metal based nanoparticles against microbes associated with diseases in aquaculture. World Journal of Microbiology and Biotechnology. 2014; 30: 2491-502.
29.  Taylor U, Barchanski A, Kues W, Barcikowski S, Rath D. Impact of metal nanoparticles on germ cell viability and functionality. Reproduction in Domestic Animals. 2012; 47: 359-68.
30.  Thomassen LC, Napierska D, Dinsdale D, Lievens N, Jammaer J, Lison D, Kirschhock CE, Hoet PH, Martens JA. Investigation of the cytotoxicity of nanozeolites A and Y. Nanotoxicology. 2012; 6(5):472-85.
31.  Victorelli FD, de Oliveira Cardoso VM, Ferreira NN, Calixto GM, Fontana CR, Baltazar F, Gremião MP, Chorilli M. Chick embryo chorioallantoic membrane as a suitable in vivo model to evaluate drug delivery systems for cancer treatment: A review. European journal of pharmaceutics and biopharmaceutics. 2020; 153: 273-84.
32.  Xiao J, Cao H, Guo S, Xiao S, Li N, Li M, Wu Y, Liu H. Long-term administration of low-dose selenium nanoparticles with different sizes aggravated atherosclerotic lesions and exhibited toxicity in apolipoprotein E-deficient mice. Chemico-Biological Interactions. 2021; 347: 109601.
علوم درمانگاهی دامپزشکی ایران
دوره 17، شماره 2 - شماره پیاپی 29
پاییز و زمستان 1402
اسفند 1402
صفحه 63-72