بررسی میزان آلودگی نمونه‌های شیر خام نشخوارکنندگان استان چهارمحال‌وبختیاری به کوکسیلا بورنتی با روش واکنش زنجیره‌ای پلیمراز آشیانه‌ای

نوع مقاله : مقاله پژوهشی

نویسندگان
1 دانشجوی دکتری باکتری‌شناسی، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد- ایران
2 گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد- ایران
3 گروه میکروب‌شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی همدان، همدان- ایران
10.22034/IJVCS.2025.14898.1103
چکیده
کوکسیلا بورنتی یک باکتری گرم منفی، داخل سلولی اجباری و عامل بیماری زئونوز تب کیو است. گاو، گوسفند و بزها منابع اصلی عفونت انسانی هستند و عامل را از طریق شیر دفع می‌کنند. این مطالعه باهدف تعیین وضعیت آلودگی به کوکسیلا بورنتی در نمونه‌های شیر خام گله‌های گاو، گوسفند و بز در استان چهارمحال‌وبختیاری انجام شد. در فاصله زمانی مهرماه 1401 تا دی‌ماه 1402، در مجموع ۲۵۰ نمونه شیر انفرادی از ۵۰ گله گاو، گوسفند و بز شیری در مناطق مختلف استان به‌طور فصلی جمع‌آوری و از نظر آلودگی به کوکسیلا بورنتی، به روش واکنش زنجیره‌ای پلیمراز آشیانه‌ای بر اساس ژن ترانسپوزونی  IS1111 مورد بررسی قرار گرفت. در مجموع ۹ نمونه (۶/3 درصد) از ۲۵۰ نمونه شیر خام شامل ۷ مورد از ۱۰۰ نمونه شیر گاوی (۷ درصد) و 2 مورد از ۷۵ نمونه شیر بزی (۶۶/2 درصد) مثبت بودند. همة ۷۵ نمونه شیر گوسفندی منفی گردیدند. ارتباط معنی‌دار بین میزان آلودگی به کوکسیلا بورنتی در نمونه‌های شیر خام مورد بررسی با نوع دام وجود داشت (042/0=Pولی در ارتباط با فصل، منطقه، سابقه ورم‌پستان و تراکم گاوداری‌ها اختلاف معنی‌داری یافت نشد. نتایج این مطالعه نشان داد که شیر خام گاو و بز می‌تواند یکی از منابع عفونت کوکسیلا بورنتی در منطقه مورد بررسی باشد.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

Survey on contamination rate of raw milk samples of ruminants in Chaharmahal and Bakhtiari province to Coxiella burnetii, using nested polymerase chain reaction method

نویسندگان English

Asghar karimian 1
Masoud Ghorbanpoor 2
Zahra Hemati 2
Leili Shokoohizadeh 3
1 Ph.D. Student of Bacteriology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord- Iran
2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord- Iran
3 Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan- Iran.
چکیده English

Coxiella burnetii is a Gram-negative, obligate intracellular bacterium and the causative agent of the zoonotic disease Q fever. Cattle, sheep, and goats are the primary sources of human infection, excreting the pathogen through milk. This study aimed to determine the prevalence of C. burnetii contamination in raw milk samples collected from cattle, sheep, and goat herds in Chaharmahal and Bakhtiari Province, Iran. Between October 2022 and January 2024, a total of 250 individual milk samples were seasonally collected from 50 dairy herds (cattle, sheep, and goats) across various regions of the province. The samples were analyzed for C. burnetii contamination using nested polymerase chain reaction (PCR) targeting the transposon gene IS1111. Totally, 9 out of 250 raw milk samples (3.6%) were positive, including 7 out of 100 cattle milk samples (7%) and 2 out of 75 goat milk samples (2.66%). All 75 sheep milk samples were found to be negative. A significant association was observed between the level of C. burnetii contamination in the raw milk samples studied and the type of livestock (P = 0.042). However, no significant differences were found concerning the season, region, history of mastitis, or dairy farm density. The findings of this study indicated that raw cow and goat milk could serve as potential sources of C. burnetii infection in the investigated area.

کلیدواژه‌ها English

Q fever
Coxiella burnetii
Milk
Cow
Sheep
Goat
Nested-PCR
1-    Ahmadizadeh C, Moosakhani F, Jamshidian M. Detection and identification of Coxiella burnetii in milk cattle of Tehran Province. Adv Biores. 2015;6(4):48–52.
2-    Berri M, Laroucau K, Rodolakis A. The detection of Coxiella burnetii from ovine genital swabs, milk, and fecal samples by the use of a single touchdown polymerase chain reaction. J Vet Microbiol. 2000;72(3-4):285–93.
3-    Borji S, Jamshidi A, Khanzadi S, Razmyar J. Detection of Coxiella burnetii and sequencing the IS1111 gene fragment in bulk tank milk of dairy herds in Iran. J Vet Sci Technol. 2014;6(2):21–8.
4-    Clark N, Soares MR. Airborne geographical dispersal of Q fever from livestock holdings to human communities: a systematic review and critical appraisal of evidence. BMC Infect Dis. 2018;18:218.
5-    Dabaja M, Greco G, Villari S, Vesco G, Bayan A, El Bazzal B, et al. Occurrence and risk factors of Coxiella burnetii in domestic ruminants in Lebanon. Comp Immunol Microbiol Infect Dis. 2019;64(4):109–16.
6-    Dhaka P, Malik VS, Yadav JP, Kumar M, Barbuddhe SB, Rawool DB. Apparent prevalence and risk factors of coxiellosis (Q fever) among dairy herds in India. PLoS One. 2020;15(9):e0239260.
7-    Fretz R, Schaeren W, Tanner M, Baumgartner A. Screening of various foodstuffs for occurrence of Coxiella burnetii in Switzerland. Int J Food Microbiol. 2007;116(3):414–8.
8-    Gami S, Antonios S, Thompson L, Chaliki P, Ammash M. Q fever endocarditis in the United States. Mayo Clin Proc. 2004;79(2):253–7.
9-    Guatteo R, Beaudeau F, Joly A, Seegers H. Shedding routes of Coxiella burnetii in dairy cows: implications for detection and control. Vet Res. 2006;37(6):827–33.
10- Guatteo R, Beaudeau F, Joly A, Seegers H. Assessing the within-herd prevalence of Coxiella burnetii milk-shedder cows using a real-time PCR applied to bulk tank milk. Zoonoses Public Health. 2007;54(5):191–4.
11- Guatto R, Seegers H, Taurel AF, Joly A, Beaudeau F. Prevalence of Coxiella burnetii infection in domestic ruminants: A critical review. Vet Microbiol Varg. 2011;149(1-2):1–16.
12- Gyuranecz M, Dénes B, Hornok S, Kovacs P, Horvath G, Jurkovich V, et al. Prevalence of Coxiella burnetii in Hungary: Screening of dairy cows, sheep, commercial milk samples, and ticks. Vector Borne Zoonotic Dis. 2013;13(8):650–3.
13- Haghi F, Zeighami H, Naderi G. Detection of major food-borne pathogens in raw milk samples from dairy bovine and ovine herds in Iran. Small Rumin Res. 2015;131:136–40.
14- Kargar M, Rashidi A, Doosti A, Ghorbani-Dalini S, Najafi A. Prevalence of Coxiella burnetii in bovine bulk milk samples in southern Iran. Comp Clin Path. 2012;22(3):665–70.
15- Karimian A, Mahzounieh MR, Ebrahimi KA. Genomic detection of Coxiella burnetii in bulk tank milk samples by Nested-PCR method in Shahrekord, Iran. Pejouhandeh. 2016;21(1):52–7.
16- Kazemeini H, Partovi R, Nazaktabar A, Shokri H. Detection of Coxiella burnetii in raw milk samples collected from dairy farms in Mazandaran province, north of Iran. J Food Saf Hyg. 2021;7(3):171–9.
17- Kersh G, Fitzpatrick K, Self J, Priestley R, Kelly A, Lash R, et al. Presence and persistence of Coxiella burnetii in the environments of goat farms associated with a Q fever outbreak. Appl Environ Microbiol. 2013;79(5):1697–703.
18- Khademi P, Ownagh A, Ataei B, Kazemnia A, Enferadi A, Khalili M, et al. Prevalence of C. burnetii DNA in sheep and goat milk in the northwest of Iran. Int J Food Microbiol. 2020;331:108716.
19- Khademi P, Ownagh A, Mardani K, Khalili M. Prevalence of Coxiella burnetii in milk collected from buffalo (water buffalo) and cattle dairy farms in Northwest Iran. Comp Immunol Microbiol Infect Dis. 2019;67:101368.
20- Khalili M, Diali HG, Mirza HN, Mosavi SM. Detection of Coxiella burnetii by PCR in bulk tank milk samples from dairy caprine herds in southeast Iran. Asian Pac J Trop Dis. 2015;5(2):119–22.
21- Kim S, Kim E, Lafferty C, Dubovi E. Coxiella burnetii in bulk tank milk samples, United States. Emerg Infect Dis. 2005;11(4):619–21.
22- Klaasen M, Roest H, van der Hoek W, Goossens B, Secka A, Stegeman A. Coxiella burnetii seroprevalence in small ruminants in The Gambia. PLoS One. 2014;9(1):e85424.
23- Klee S, Tyczka J, Ellerbrok H, Franz T, Linke S, Baljer G. Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii. BMC Microbiol. 2006;6:2.
24- Loftis A, Priestley R, Massung R. Detection of Coxiella burnetii in commercially available raw milk from the United States. Foodborne Pathog Dis. 2010;7(12):1453–6.
25- Lyytikainen O, Ziese T, Schwartlander B, Matzdorff P, Kuhnhen C, Jager C, et al. An outbreak of sheep-associated Q fever in a rural community in Germany. Eur J Epidemiol. 1998;14(2):193–9.
26- Maurin M, Raoult D. Q fever. Clin Microbiol Rev. 1999;12(4):518–53.
27- Martinov S. Studies on mastitis in sheep caused by Coxiella burnetii. Biotechnol Biotechnol Equip. 2007;21(4):484–90.
28- Mohabati Mobarez A, Mostafavi E, Khalili M, Esmaeili S. Identification of Coxiella burnetii in raw milk of livestock animals in Iran. Int J Microbiol. 2021; doi:10.1155/2021/6632036.
29- Nokhodian Z, Feizi A, Moradi A, Yaran M, Hoseini S, Ataei B, et al. Detection and risk factors of Coxiella burnetii infection in dairy cattle based on bulk tank milk samples in central Iran. Prev Vet Med. 2016;134:139–44.
30- Pandit P, Hoch T, Ezanno P, Beaudeau F, Vergu E. Spread of Coxiella burnetii between dairy cattle herds in an enzootic region: Modelling contributions of airborne transmission and trade. Vet Res. 2016; doi:10.1186/s13567-016-0330-4.
31- Parisia R, Fraccalvieri M, Cafiero A, Miccolupo I, Padalino C, Montagna C, et al. Diagnosis of Coxiella burnetii-related abortion in Italian domestic ruminants using single-tube nested PCR. Vet Microbiol. 2006;118(1-2):101–6.
32- Parker N, Barralet J, Bell A. Q fever. Lancet. 2006;367(9511):679–88.
33- Plummer P, McClure J, Menzies P, Morley P, Van den Brom R, Van Metre D. Management of Coxiella burnetii infection in livestock populations and the associated zoonotic risk: a consensus statement. J Vet Intern Med. 2018;32(5):1481–94.
34- Porten K, Rissland J, Tigges A, Broll S, Hopp W, Lunemann M, et al. A super-spreading ewe infects hundreds with Q fever at a farmers' market in Germany. BMC Infect Dis. 2006;6:147.
35- Rahimi E, Ameri M, Karim G, Doosti A. Prevalence of Coxiella burnetii in bulk milk samples from dairy bovine, ovine, caprine, and camel herds in Iran as determined by polymerase chain reaction. Foodborne Pathog Dis. 2011;8(2):307–10.
36- Rahimi E, Torki Z, Doosti A. An assay to determine the seasonal prevalence of Coxiella burnetii in cow milk using nested PCR. J Microbial World. 2010;3(1):56.
37- Raoult D, Marrie T, Mege J. Natural history and pathophysiology of Q fever. Lancet Infect Dis. 2005;5(4):219–26.
38- Raoult D, Marrie T. Q fever. Clin Infect Dis. 1995;20(3):489–95.
39- Rodolakis A, Berri M, Hechard C, Caudron C, Souriau A, Bodier C. Comparison of Coxiella burnetii shedding in milk of dairy bovine, caprine, and ovine herds. J Dairy Sci. 2007;90(12):5352–60.
40- Roest H, Tilburg J, Van der Hoek W, Vellema P, Van Zijderveld F, Klaassen C. The Q fever epidemic in The Netherlands: history, onset, response, and reflection. J Epidemiol Infect. 2011;139(1):1–12.
41- Sadiki V, Gcebe N, Mangenas ML, Ngoshe YB, Adesiyun AA. Prevalence and risk factors of Q fever (Coxiella burnetii) in cattle on farms of Limpopo province, South Africa. Front Vet Sci. 2023; doi: 10.3389/fvets.2023.1101988.
42- Schimmer B, Luttikholt S, Hautvast JL, Graat EA, Vellema P, van Duynhoven YT. Seroprevalence and risk factors of Q fever in goats on commercial dairy goat farms in the Netherlands, 2009-2010. BMC Vet Res. 2011;7:1–14.
43- Scott GH, Williams JC. Susceptibility of Coxiella burnetii to chemical disinfectants. Ann N Y Acad Sci. 1990;590:291–6.
44- Senay S, Zulal O, Ufuk D, Biray O. The seroprevalence of Coxiellosis in farmers and cattle in Erzurum. Turk J Vet Anim Sci. 2006;30(1):71–5.
45- Stein A, Raoult D. Detection of Coxiella burnetii by DNA amplification using polymerase chain reaction. J Clin Microbiol. 1992;30(9):2462–6.
46- Svraka S, Toman R, Skultet L, Slaba K, Homan WL. Establishment of a genotyping scheme for Coxiella burnetii. FEMS Microbiol Lett. 2006;254:268–74.
47- Turra M, Chang G, Whybrow D, Higgins G, Qiao M. Diagnosis of acute Q fever by PCR on sera during a recent outbreak in rural South Australia. Ann N Y Acad Sci. 2006;1078:5669–79.
48- van der Hoek W, Hunink J, Vellema P, Droogers P. Q fever in the Netherlands: the role of local environmental conditions. Int J Environ Health Res. 2011;21(6):441–51.
49- Wikipedia contributors. Chaharmahal and Bakhtiari province. Wikipedia, The Free Encyclopedia. Last modified January 9, 2025. Available from: https://en.wikipedia.org/wiki/Chaharmahal and Bakhtiari province.
50- Yesim Can H, Elmal M, Karag A. Detection of Coxiella burnetii in cows’, goats’, and ewes’ bulk milk samples using polymerase chain reaction (PCR). Mljekarstvo. 2015;65(1):26–31.