تشخیص فاکتورهای حدت مایکوپلاسما‌ گالی‌سپتیکوم (MG)، جدا شده از جوجه های مبتلا به کمپلکس بیماری های تنفسی، با استفاده از تکنیک Real- time PCR High-Resolution Melting Curve

نوع مقاله : مقاله پژوهشی

نویسندگان
آرش رمضانپور شاهی 1
عبدالکریم زمانی مقدم 1
محمدرضا محزونیه 2
1 گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران
2 گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران
10.22034/ijvcs.2026.14740.1084
چکیده
مایکوپلاسما‌ گالی‌سپتیکوم (MG) یک پاتوژن باکتریایی است که باعث بیماری مزمن تنفسی (CRD) در طیور می شود. CRD نه تنها میزان بهره وری در مزارع مرغداری را کاهش می‌دهد بلکه منجر به زیان اقتصادی قابل توجهی در صنعت طیور می‌شود. روش‌های مولکولی به عنوان ابزاری قابل اعتماد برای تشخیص این عفونت باکتریایی ظهور کرده‌اند. هدف از این مطالعه شناسایی فاکتورهای حدت مایکوپلاسما‌ گالی‌سپتیکوم جدا شده از جوجه‌های مبتلا به بیماری های تنفسی با استفاده از تکنیک Real-time PCR High-Resolution Melting curve (HRM) بود. به طورکلی 50 مزرعه مرغ گوشتی در این مطالعه وارد شدند. از هر مزرعه 20 نمونه جمع آوری شد. استخراج DNA با استفاده از کیت استخراج DNA CTB i-genomic انجام شد. از 50 مزرعه‌ای که نمونه‌ها جمع آوری شد، چهار مزرعه با استفاده از روش Real-time PCR به عنوان مایکوپلاسما مثبت شناسایی شدند. منحنی ذوب با وضوح بالا Real-time PCR انجام شد و داده‌های به دست آمده با استفاده از سیستم PCR max تجزیه و تحلیل شدند. نتایج PCR نشان داد که ژن‌های pvpA، crmA و gapA در این نمونه‌ها وجود داشتند، نتایج ترسیم درخت فیلوژنتیک با نمونه‌های مشابه در مطالعات قبلی مقایسه شد. تجزیه و تحلیل HRM تنوع مولکولی بین نمونه‌های جدا شده را نشان می‌دهد. نتایج این مطالعه نشان داد که استفاده از HRM Real-time PCR روشی قابل اعتماد برای شناسایی و بررسی وجود فاکتورهای حدت مرتبط با پاتوژن مایکوپلاسما است.
کلیدواژه‌ها
مایکوپلاسما گالی‌سپتیکوم
بیماری مزمن تنفسی
طیور
ژن حدت
HRM Real-time PCR
موضوعات

عنوان مقاله English

Detection of virulence factors of Mycoplasma gallisepticum (MG), isolated from broilers with chronic respiratory disease, using Real-time PCR High-Resolution Melting Curve technique

نویسندگان English

Arash Ramezanpour shahi 1
Abdolkarim Zamani Moghadam 1
Mohammadreza Mahzounieh 2
1 Department of clinical sciences, Faculty of veterinary, Shahrekord university, Shahrekord, Iran
2 Department of pathobiology Faculty of Veterinary, Shahrekord University, Shahrekord, Iran
چکیده English

Mycoplasma gallisepticum (MG) is a bacterial pathogen that causes chronic respiratory disease (CRD) in poultry. CRD not only reduces productivity in poultry farms but also leads to significant economic losses in the poultry industry. Molecular methods have emerged as a reliable tool for the diagnosis of this bacterial infection. The aim of this study was to identify virulence factors of Mycoplasma gallisepticum isolated from chickens with respiratory diseases using Real-time PCR High-Resolution Melting curve (HRM) technique. In general, 50 broiler farms were included in this study. 20 samples were collected from each farm. DNA extraction was performed using CTB i-genomic DNA extraction kit. From 50 farms where samples were collected, four farms were identified as Mycoplasma positive using Real-time PCR method. The melting curve was performed with high-resolution real-time PCR and the obtained data were analyzed using the PCR max system. PCR results showed that pvpA, crmA and gapA genes were present in these samples, the results of phylogenetic tree drawing were compared with similar samples in previous studies. HRM analysis reveals molecular diversity between isolates. The results of this study showed that the use of HRM Real-time PCR is a reliable method to identify and investigate the presence of virulence factors related to the Mycoplasma pathogen.

کلیدواژه‌ها English

Mycoplasma gallisepticum
chronic respiratory disease
Poultry
virulence gene
HRM Real-time PCR
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علوم درمانگاهی دامپزشکی ایران
دوره 18، شماره 1 - شماره پیاپی 30
بهار و تابستان 1403
مرداد 1403
صفحه 35-45