تأثیر عصاره پوست انار بر سمیت مغزی ناشی از کادمیم در ماهی کپور معمولی (Cyprinus carpio)؛ ارزیابی هیستوپاتولوژیک

نوع مقاله : مقاله پژوهشی

نویسندگان
حسین جعفرزاده 1
سوده علیدادی 2
داور شاهسونی 3
1 دانش‌آموخته دکترای عمومی دامپزشکی، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، مشهد، ایران
2 Ferdowsi University of Mashhad
3 گروه بهداشت مواد غذایی و آبزیان، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، مشهد، ایران
10.22034/ijvcs.2026.14894.1091
چکیده
این پژوهش با هدف بررسی اثرات حفاظتی عصاره پوست انار بر بافت مغز در مسمومیت تجربی با کادمیم در ماهی کپور انجام شد. 150 ماهی کپور معمولی در سه تکرار به صورت تصادفی در 5 گروه تقسیم شده و به مدت چهار هفته مورد‌بررسی قرار گرفتند. گروه شاهد بدون کادمیم، گروه کادمیم میزان 0/5 میلی‌گرم بر لیتر کادمیم کلرید؛ گروه کادمیم+عصاره1%، علاوه بر 0/5 میلی‌گرم بر لیتر کادمیم کلرید در آب، مقدار 0/35 گرم عصاره پوست انار (1 درصد وزن غذا)؛ گروه کادمیم+عصاره2%، علاوه بر کادمیم، 0/7 گرم عصاره پوست انار (2 درصد وزن غذا)؛ و گروه کادمیم+عصاره4% نیز علاوه بر کادمیم، 1/4 گرم عصاره پوست انار (4 درصد وزن غذا) را در چهار وعده غذایی به‌طور مساوی دریافت کردند. پس از اتمام دوره تیمار به مدت چهار هفته، از هر گروه 6 ماهی به‌طور تصادفی انتخاب شده و نمونه‌های بافتی از مغز آن‌ها گرفته، در فرمالین 10 درصد بافر خنثی پایدار شده، و با هماتوکسیلین-ائوزین رنگ شدند. تغییرات هیستوپاتولوژیکی مشاهده شده در بافت مغز گروه کادمیم شامل پرخونی، خونریزی، دژنراسیون و نکروز نورون‌ها، ادم و حالت واکوئولاسیون، و گلیوز بودند. در مقادیر مختلف عصاره پوست انار به‌ویژه 2 و 4 درصد کاهش تغییرات مشاهده شد ولی تنها در گروه کادمیم+عصاره4% نسبت به گروه کادمیم معنی‌دار بود (0/05>p). به‌طورکلی می‌توان چنین نتیجه‌گیری کرد که عصاره 4 درصد پوست انار اثرات محافظتی قابل‌توجهی بر بافت مغز در مسمومیت تجربی با کادمیم در ماهی کپور دارد.
کلیدواژه‌ها
کادمیم کلرید
عصاره پوست انار
هیستوپاتولوژی
مغز
ماهی کپور معمولی
موضوعات

عنوان مقاله English

Effect of pomegranate peel extract on cadmium-induced brain toxicity in common carp (Cyprinus carpio); Histopathological evaluation

نویسندگان English

Hossein Jafarzadeh 1
Soodeh Alidadi 2
Davar Shahsavani 3
1 Graduated of Veterinary Medicine, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
2 Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad
3 Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده English

This research was conducted to investigate the protective effects of pomegranate peel extract on brain tissue in experimental cadmium poisoning in common carp. 150 common carp were randomly divided into 5 groups in triplicate and evaluated for four weeks. The control group did not receive cadmium, the cadmium group received 0.5 mg/L of cadmium chloride; the cadmium+1% extract group received 0.35 g of pomegranate peel extract (1% of food weight) in addition to 0.5 mg/L of cadmium chloride in water; the cadmium+2% extract group received 0.7 g of pomegranate peel extract (2% of food weight) in addition to cadmium; and the cadmium+4% extract group received 1.4 g of pomegranate peel extract (4% of food weight) in addition to cadmium equally in four meals. After the four-week treatment period, six fish from each group were randomly selected and tissue samples were taken from their brains, fixed in 10% neutral buffered formalin, and stained with hematoxylin and eosin. Histopathological changes observed in the brain tissue of the cadmium group included hyperemia, hemorrhage, degeneration and necrosis of neurons, edema and vacuolation, and gliosis. Different amounts of pomegranate peel extract, especially 2% and 4%, reduced changes, but only the cadmium+4% extract was significant compared to the cadmium group (p<0.05). In general, it can be concluded that 4% pomegranate peel extract has significant protective effects on brain tissue in experimental cadmium poisoning in common carp.

کلیدواژه‌ها English

Cadmium chloride
Pomegranate peel extract
Histopathology
Brain
Common carp
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علوم درمانگاهی دامپزشکی ایران
دوره 19، شماره 2 - شماره پیاپی 33
پاییز و زمستان 1404
اسفند 1404
صفحه 37-46