تأثیر مکمل هیدروکسی کلرید روی بر فراسنجه‌های فشار خون ریوی، ذخیره‌ی عنصر روی و استحکام استخوان در جوجه‌های گوشتی در شرایط پرورش ارتفاع بالا

نوع مقاله : مقاله پژوهشی

نویسندگان
1 گروه علوم دامی، دانشکده کشاورزی، دانشگاه شهرکرد، شهرکرد، ایران
2 گروه علوم دامی، دانشکده کشاورزی، دانشگاه شهرکرد، شهرکرد، ایران.
3 گروه علوم دامی، آموزشگاه کشاورزی شهید باهنر، پاکدشت، تهران، ایران
10.22034/ijvcs.2026.14832.1086
چکیده
هدف این مطالعه، ارزیابی تأثیر سطوح مختلف هیدروکسی کلرید روی (HCZ) بر فراسنجه های مرتبط با فشار خون ریوی، ذخیره عنصر روی و استحکام استخوان درشت نی جوجه‌های گوشتی در شرایط محیطی با ارتفاع بالا از سطح دریا (2100 متر) بوده است. در این پژوهش از 200 قطعه جوجه خروس سویه راس 308 از سن 23 تا 42 روزگی برای اجرای یک طرح کاملاً تصادفی در قالب 4 تیمار و 5 تکراراستفاده شد. تیمارهای آزمایشی شامل ﺟﻴﺮﻩ ﭘﺎﻳﻪ و 25، 50 و 75 میلی‌گرم HCZ در هر کیلوگرم خوراک پایه بود که به جیره شاهد ﺍفزوده شد. مصرف HCZ باعث کاهش نسبت RV به TV ، افزایش غلظت نیتریک اکساید، کاهش هماتوکریت و نسبت هتروفیل به لنفوسیت گردید (05/0P<). همچنانکه افزایش ذخیره‌ی عنصر روی در ماهیچه سینه، کبد و استخوان درشت نی مشاهده گردید (05/0P<)، افزایش ضربه شکست استخوان بیانگر افزایش استحکام استخوان درشت نی بود (05/0P<). به‌طورکلی نتایج نشان‌داد مصرف 75 میلی‌گرم HCZ باعث بهبود پاسخ‌های فیزیولوژیک و ایمنی، افزایش ذخیره عنصر روی در بافت استخوان، سینه و کبد، بهبود فراسنجه‌های مربوط به رشد و استحکام استخوان درشت نی و عملکرد قلبی ریوی جوجه‌های گوشتی تحت شرایط هیپوکسی گردید. بنابراین سطح 75 میلی‌گرم HCZ جهت مصرف در سطح مزارع پرورشی قابل توصیه می باشد.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

Effect of Zinc Hydroxychloride Supplementation on Pulmonary Hypertension Parameters, Zinc Retention, and Bone Strength in Broiler Chickens Under High-Altitude Rearing Conditions

نویسندگان English

Amir Parvaresh 1
Behnam Ahmadipour 2
Samira Abaszadeh 1
Amir Afshar Bakeshlo 3
1 4 Department of Animal Science, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran
2 Department of Animal Science, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran.
3 Department of Animal Sciences, Shahid Bahonar Agriculture School, Pakdasht, Tehran, Iran
چکیده English

This study aimed to evaluate the effect of different levels of zinc hydroxychloride (HCZ) on pulmonary hypertension-related parameters, zinc element storage, and tibia bone strength in broiler chickens under high-altitude environmental conditions (2100m). In this research, 200 Ross 308 male broiler chicks from 23 to 42 days of age were used for a completely randomized design in 4 treatments and 5 replicates. The experimental treatments included a basal diet and 25, 50, and 75 mg of HCZ per kg of basal feed, which were added to the control diet. HCZ consumption led to a decrease in the RV to TV ratio, an increase in nitric oxide concentration, a decrease in hematocrit, and a decrease in the heterophil to lymphocyte ratio (P<0.05). As the retention of zinc in breast muscle, liver, and tibia bone increased (P<0.05), the increased bone fracture force indicated an increase in tibia bone strength (P<0.05). Overall, the results showed that consuming 75 mg of HCZ improved physiological and immune responses, increased zinc storage in bone tissue, breast, and liver, improved growth parameters and tibia bone strength, and cardiopulmonary performance of broiler chickens under hypoxic conditions. Therefore, a level of 75 mg HCZ is recommended for use in poultry farms.

کلیدواژه‌ها English

High altitude
Bone strength
Pulmonary hypertension
Zinc hydroxychloride
1.      Afshar Bakeshlo A, Ahmadipour B, Khajali F, Pirany N. Investigating the effect of different sources and levels of zinc on the immune performance and antioxidant capacity of laying hens. Iran J Clinic Sci. 2024;17(2):81-90.
2.      Alcantara EH, Lomeda RAR, Feldmann J, Nixon GF, Beattie JH, Kwun IS. Zinc deprivation inhibits extracellular matrix calcification through decreased synthesis of matrix proteins in osteoblasts. Mol Nutr Food Res. 2011;55(10):1552-1560.
3.      Aviagen, 2022. Aviagen Group Ross 308 Broiler: Nutrition Specifications. Available online: https://eu.aviagen.com/assets/Tech_Center/Ross_Broiler/RossBroilerNutritionSpecs2019-EN.pdf
4.      Behrooj N, Khajali F, Hassanpour H. Feeding reduced‐protein diets to broilers subjected to hypobaric hypoxia is associated with the development of pulmonary hypertension syndrome. Br Poult Sci. 2012;53:658-664.
5.      Chawalitjinda O, Songserm O, Pongmanee K, Ruangpanit Y. Effect of zinc hydroxychloride supplementation on growth performance and zinc deposition in broiler chickens. J Mahanakorn Vet Med. 2021;16(1):11-21.
6.      Chen J, Jiang C, Hu X, Zhang Y, Gao X, Guo X, Liu P. Mechanism of pulmonary arterial vascular cell dysfunction in pulmonary hypertension in broiler chickens. Avian Pathology. 2025;54(5):548–559. doi:10.1080/03079457.2025.2480802.
7.      Fathi M, Zarrinkavyani K, Biranvand Z, Al Abd MA. Effect of Phloretin on Antioxidant Status, Inflammatory Cytokines, Blood and Biochemical Indices, Cecal Microbial Load, and Growth Performance in Broiler Chickens Subjected to Pulmonary Arterial Hypertension. Poult Sci. 2025;105265.
8.      Ghosh A, Kumar A, Biswas G. Exponential population growth and global food security: challenges and alternatives. In: Bioremediation of emerging contaminants from soils. 2024:1-20.
9.      Guo D, Zhang J, Han Y, Cui L, Wang H, Wang K, et al. Transcriptomic study on the lungs of broilers with ascites syndrome. Animal. 2023;13(1):175.
10.  Hanson ZD, Mirshahidi H, Brothers J, Mirshahidi S, Pham B, Samaeekia R, et al. Hemoglobin response to zinc supplementation in patients with zinc deficiency and chronic anemia. Blood. 2023;142:5222.
11.  Hassanpour H, Yazdani A, Khabir Soreshjani K, Asgharzadeh S. Evaluation of endothelial and inducible nitric oxide synthase genes expression in the heart of broiler chickens with experimental pulmonary hypertension. Br Poult Sci. 2009;50(6):725-732.
12.  Izadinia M, Nobakht M, Khajali F, Faraji M, Zamani F, Qujeq D, et al. Pulmonary hypertension and ascites as affected by dietary protein source in broiler chickens reared in cool temperature at high altitudes. Anim Feed Sci Technol. 2010;155(2-4):194-200.
13.  Khajali F, Tahmasebi M, Hasanpour H, Akbari MA, Qujeeq D, Wideman RF. Effects of supplementation of canola meal-based diets with arginine on performance, plasma nitric oxide, and carcass characteristics of broiler chickens grown at high altitude. Poult Sci. 2011;90:2287-2294.
14.  Leisure NJ, Jackson CC, Huang M, Moore TB, Steward FA. Micronutrient supplement [United States Patent US 8.No]. 20130064963; 2014.
15.  Lima L, Moraes T, Morais L, de Amorim M, Veríssimo T, Dos Santos J, et al. Effect of zinc level and the thermal environment on the zootechnical performance and tibia-breaking strength of Japanese quails. Front Vet Sci. 2025;11:1467487.
16.  Liu ZH, Lu L, Wang RL, Lei HL, Li SF, Zhang LY, et al. Effects of supplemental zinc source and level on antioxidant ability and fat metabolism-related enzymes of broilers. Poult Sci. 2015;94(11):2686–2694.
17.  Lu L, Wang RL, Zhang ZJ, Steward FA, Luo X, Liu B. Effect of dietary supplementation with copper sulfate or tribasic copper chloride on the growth performance, liver copper concentration of broilers fed in floor pens, and stabilities of vitamin E and phytase in feed. Biol Trace Elem Res. 2010;138:181-9.
18.  Marreiro DD, Cruz KJ, Morais JB, Beserra JB, Severo JS, De Oliveira AR. Zinc and oxidative stress: current mechanisms. Antioxidants (Basel). 2017;6(2):24.
19.  Miles RD, O’Keefe SF, Henry PR, Ammerman CB, Luo XG. The effect of dietary supplementation with copper sulfate or tribasic copper chloride on broiler performance, relative copper bioavailability, and dietary prooxidant activity. Poult Sci. 1998;77:416-25.
20.  Moreno J, Merino S, Martínez J, Sanz J, Arriero E. Heterophil/lymphocyte ratios and heat-shock protein levels are related to growth in nestling birds. Ecoscience. 2002;9(4):434-439.
21.  National Research Council. Nutrient Requirements of Poultry. 9th rev ed. Washington, DC: Natl Acad Press; 1994.
22.  Nguyen HT, Morgan N, Roberts JR, Wu SB, Swick RA, Toghyani M. Zinc hydroxychloride supplementation improves tibia bone development and intestinal health of broiler chickens. Poult Sci. 2021;100(8):101254.
23.  Olukosi OA, van Kuijk S, Han Y. Copper and zinc sources and levels of zinc inclusion influence growth performance, tissue trace mineral content, and carcass yield of broiler chickens. Poult Sci. 2018;97(11):3891–3898.
24.  Park SY, Birkhold SG, Kubena LF, Nisbet DJ, Ricke SC. Review on the role of dietary Zinc in poultry nutrition, immunity and reproduction. Biol Trace Elem Res. 2004;101:147-163.
25.  Perez V, Shanmugasundaram R, Sifri M, Parr TM, Selvaraj RK. Effects of hydroxychloride and sulfate form of zinc and manganese supplementation on superoxide dismutase activity and immune responses post lipopolysaccharide challenge in poultry fed marginally lower doses of zinc and manganese. Poult Sci. 2017;96(12):4200–4207.
26.  Rowland JR, Harms RH, Wilson HR, Ross IJ, Fry JL. Breaking strength of chick bones as an indication of dietary calcium and phosphorus adequacy. Proc Soc Exp Biol Med. 1967;126(2):399-401.
27.  Sadr V, Nguyen HTT, Pineda L, Han Y, Toghyani M. Synergistic Effects of Hydroxychloride and Organic Zinc on Performance, Carcass Characteristics, Liver and Tibia Mineral Profiles of Broiler Chickens. Biol Trace Elem Res. 2024;1-10.
28.  Santos LFJ, Gonçalves AM, Neira LM, Nakagi VS, Macari M, Laurentiz AC, Pizauro JM. Effects of Supplementation of Zinc, Manganese, or Copper and Different Phytase Levels in Serum and Bone Acid and Alkaline Phosphatases of Broiler Chicks. Braz J Poult Sci. 2023;25(02):eRBCA-2022.
29.  Shelton JL, Southern LL. Interactive Effect of Zinc, Copper and Manganese in Diets for Broilers. Int J Poult Sci. 2007;6:466-9.
30.  Sim JY. Nitric oxide and pulmonary hypertension. Korean J Anesthesiol. 2010;58(1):4-14.
31.  Stiles LI, Ferrao K, Mehta KJ. Role of zinc in health and disease. Clin Exp Med. 2024;24(1):38.
32.  Štofaníková J, Šály J, Molnár L, Sesztáková E, Vrabec V. The mechanical properties of broiler chicken bones affected by different dietary zinc levels. Afr J Biotechnol. 2012;11(20):4681-6.
33.  Szőllősi L, Béres E, Szűcs I. Effects of modern technology on broiler chicken performance and economic indicators–a Hungarian case study. Ital. J. Anim. Sci. 2021;20(1):188-194.
34.  Tomaszewska E, Muszynski S, Dobrowolski P, Kwiecien M, Winiarska-Mieczan A, Swietlicka I, Wawrzyniak A. Effect of zinc level and source (zinc oxide vs. zinc glycine) on bone mechanical and geometric parameters, and histomorphology in male Ross 308 broiler chicken. Rev Bras Cienc Avic. 2017;19:159–70.
35.  Wenqiang M, Haihua N, Feng J, Wang Y, Feng Ji. Effects of Zinc glycine chelate on oxidative Stress, content of trace elements and intestinal morphology in broilers. Biol Trace Elem Res. 2010;142:564-6.
36.  Yamaguchi M. Role of zinc in bone metabolism and preventive effect on bone disorder. Biomed Res Trace Elem. 2007;18(4):346-66.
37.  Zhang B, Guo Y. Beneficial effects of tetrabasic zinc chlo ride for weanling piglets and the bioavailability of zinc in tetraba sic form relative to ZnO. Anim Feed Sci Technol. 2007; 135(1–2):75–85 26.
38.  Zhang Y, Xie L, Ding X, Wang Y, Xu Y, Li D, et al. Mechanisms underlying the protective effect of maternal zinc (ZnSO4 or Zn-Gly) against heat stress-induced oxidative stress in chicken embryo. Antioxid. 2022; 11(9): 1699.