تعیین میزان مقاومت آنتی‌بیوتیکی ایزوله‌های اشریشیا کلی جدا شده از طیور درگیر بیماری کلی‌باسیلوز در مرغداری‌های گوشتی استان چهارمحال و بختیاری

نوع مقاله : مقاله پژوهشی

نویسندگان
سید ستار توحیدی فر 1
سید محمد ضیایی 2
عبدالکریم زمانی مقدم 3
سید رضا ابطحی 2
کاظم نوروزی 4
1 گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران
2 گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران.
3 گروه علوم درمانگاهی، دانشکده دامپرشکی، دانشگاه شهرکرد، شهرکرد، ایران.
4 دانشجوی دکتری تخصصی رشته فناوری تولیدمثل در دامپزشکی، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران
10.22034/ijvcs.2026.15029.1099
چکیده
عامل ایجاد کلی‌باسیلوز، اشریشیا کلی است که از طریق آب، دان، هوای آلوده، وسایل پرورشی آلوده و یا از مادر به تخم‌مرغ و نهایتاً جوجه منتقل می‌شود. ﺩﺭ ﺳﻪ ﺩﻫﻪ اخیر، ﻣﻮﺍﺭﺩ ﻣﺘﻌﺪﺩی ﺍﺯ ﻣﻘﺎﻭﻣﺖ ﺑﻪ ﻓﻠﻮﺭﻓﻨﻴﻜﻞ و ﻛﻴﻨﻮﻟﻮﻥ‌ها ﺩﺭ ﺍﻧﺴﺎﻥ ﻭ ﺩﺍﻡ ﮔﺰﺍﺭﺵ ﺷﺪﻩ ﻭ ﻳﻜﻲ ﺍﺯ ﺩﻻﻳﻞ ﺑﺮﻭﺯ ﻣﻘﺎﻭﻣﺖ ﺩﺭ ﺍﻧﺴﺎﻥ، ﺍﺳﺘﻔﺎﺩﻩ ﮔﺴﺘﺮﺩﻩ ﺍﻳﻦ ﺩﺍﺭﻭﻫﺎ ﺩﺭ ﭘﺮﻭﺭش ﻃﻴﻮﺭ ﺫﻛﺮ گردیده است. هدف ما در مطالعه حاضر، بررسی میزان مقاومت آنتی‌بیوتیکی جدایه‌های اشریشیا کلی جدا شده از فارم‌های گوشتی در استان چهارمحال و بختیاری است. در این تحقیق، 204 نمونه از گله‌های درگیر با کلی‌باسیلوز که حداقل 24 ساعت قبل آنتی‌بیوتیک مصرف نکرده بودند و در سال‌های بین 1398-1403 به کلینیک دانشکده دامپزشکی دانشگاه شهرکرد مراجعه کردند، مورد بررسی و آزمون آنتی‌بیوگرام قرار گرفتند. با توجه به نتایج به‌دست‌آمده، مقاومت 100 درصدی به اگزاسیلین، 06/97% مقاومت به اریترومایسین، 18/91% مقاومت به اکسی‌تتراسایکلین، 76/86% مقاومت به سولتریم، 88/80% مقاومت به فلومکوئین، 90/79% مقاومت به دای‌فلوکساسین، 94/77% مقاومت به دانوفلوکساسین، 98/75% مقاومت به آمپی‌سیلین، 06/72% مقاومت به انروفلوکساسین، 14/68% مقاومت به سیپروفلوکساسین، 28/61% مقاومت به فلورفنیکل، 69/40% مقاومت به لینکواسپکتین و 95/27% مقاومت به فسفومایسین مشاهده شد. بر این اساس، فسفومایسین، لینکواسپکتین و فلورفنیکل، به ترتیب، آنتی‌بیوتیک‌هایی هستند که دارای کمترین مقاومت در مقایسه با سایر آنتی‌بیوتیک‌ها بوده است. یافته‌های مطالعه حاضر حاکی از فراوانی میزان مقاومت آنتی‌بیوتیکی در گله‌های مبتلا به کلی‌باسیلوز است که این امر، خطری جدی برای صنعت طیور کشور و بهداشت عمومی محسوب می‌شود.
کلیدواژه‌ها
آنتی‌بیوگرام
مقاومت آنتی‌بیوتیکی
طیور گوشتی
کلی‌باسیلوز
موضوعات

عنوان مقاله English

Investigating the Antibiotic Resistance of Escherichia coli Isolated from Poultry Infected with Colibacillosis in Broiler Farms of Chaharmahal and Bakhtiari Province

نویسندگان English

Seyyed Sattar Tohidifar 1
Seyyed Mohammad Ziaei 2
Abdolkarim Zamani Moghadam 3
Seyyed Reza Abtahi 2
Kazem Norouzi 4
1 Department of Animal Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
3 Department of Clinical Sciences,, Faculty of Veterinary Medicine,, Shahrekord University, Shahrekord, Iran.
4 PhD Student in Veterinary Reproductive Biotechnology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
چکیده English

The causative agent of colibacillosis is Escherichia coli, transmitted through water, manure, contaminated air, contaminated breeding equipment, or from the mother to the egg, and finally to the chick. Over the last three decades, several cases of resistance to florfenicol and quinolones have been reported in humans and livestock. One of the reasons for the emergence of resistance in humans is the widespread use of these drugs in poultry farming.
This study aims to investigate antibiotic resistance levels in Escherichia coli isolates from poultry farms in Chaharmahal and Bakhtiari province. In this research, 204 samples from broiler farms that visited the clinic of Shahrekord University Veterinary School between 2019 and 2024 were examined and subjected to antibiogram testing.
According to the obtained results, resistance was observed as follows: oxacillin 100.00%, erythromycin 97.06%, oxytetracycline 91.18%, trimethoprim-sulfamethoxazole 86.76%, flumequine 80.88%, difloxacin 79.90%, danofloxacin 77.94%, ampicillin 75.98%, enrofloxacin 72.06%, ciprofloxacin 68.14%, florfenicol 61.28%, lincospectin 40.69%, and fosfomycin 27.95%.
Based on these findings, Fosfomycin, Lincospectin, and Florfenicol are antibiotics with the least resistance compared to others. Our results indicate a concerning level of antibiotic resistance in colibacillosis infection; This poses a serious risk to both the poultry industry in the country and public health.

کلیدواژه‌ها English

Antibiogram
Antibiotic Resistance
Broiler
Collibacillosis
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علوم درمانگاهی دامپزشکی ایران
دوره 20، شماره 1 - شماره پیاپی 34
بهار و تابستان 1405
شهریور 1405
صفحه 55-65