تجزیه و تحلیل فیلوژنتیکی ویروس بیماری منقار و پر طوطی سانان (BFDV) در اصفهان

نوع مقاله : مقاله پژوهشی

نویسندگان
مهران تاجمیر ریاحی 1
عبدالکریم زمانی مقدم 1
محمدرضا محزونیه 2
1 دانشگاه شهرکرد
2 دانشگاه آدلاید استرالیا
10.22034/ijvcs.2024.14772.1085
چکیده
ویروس بیماری منقار و پر (BFDV)، متعلق به خانواده Circoviridae و از لحاظ ساختاری دارای هفت فریم خوانش باز (ORFs) است که تنها دو ORF C1 و ORF V1، بیشتر تحت تاثیر تغییرات ژنتیکی قرار دارند. BFDV مستعد جهش هایی است که به سیرکوویروس اجازه می دهد تا حدت خود را حفظ کند. از آنجایی که تفاوت هایی در سرعت تکثیر بین توالی های Rep ، Cap و بین ژنی وجود دارد، ارزیابی کامل کل توالی ژنوم BFDV برای تعیین همه فرصت های نوترکیبی اهمیت زیادی دارد. در این مطالعه از طوطی های ارجاعی به کلینیک های دامپزشکی اصفهان(2021 - 2022) ، 300 طوطی مشکوک به PBFD از گونه های عروس هلندی، طوطی سبز و طوطی خاکستری آفریقایی برای نمونه گیری انتخاب شدند. تقریبا 4 تا 8 پر از هر پرنده که علائم بیماری را نشان می داد جدا گردید و DNA کل از نمونه های بالینی جدا شد. از میان موارد مثبت، 5 نمونه انتخاب و توالی نوکلئوتیدی آنها با استفاده از روش Sanger بر روی محصولات PCR تعیین شد. مقایسه توالی نوکلئوتیدی نمونه ها نشان داد که این 5 ویروس به 3 شاخه تقسیم می شوند. توالی های نوکلئوتیدی از نمونه های عروس هلندی و نمونه های طوطی خاکستری آفریقایی در یک شاخه با 97% شباهت به یکدیگر قرار گرفتند و با نمونه های طوطی خاکستری آفریقایی و باجریگار جدا شده در عربستان سعودی در سال 2017 در یک شاخه قرار گرفتند. توالی نوکلئوتیدی طوطی سبز بیشترین شباهت نوکلئوتیدی را به نمونه های جدا شده از طوطی سبز و سایر طوطی ها در عربستان سعودی و آفریقای جنوبی نشان داد.
کلیدواژه‌ها
ویروس پر و منقار طوطی سانان
فیلوژنی
جهش
توالی یابی
موضوعات

عنوان مقاله English

Phylogenetic analysis of psittacine beak and feather disease virus (BFDV) in Isfahan

نویسندگان English

Mehran Tajmir Riahi 1
Abdolkarim Zamani Moghadam 1
Mohammad Reza Mahzonieh 2
1 Shahrekord University
2 Department of Microbiology, Faculty of Veterinary Medicine, Adelaide University, Adelaide- Australia
چکیده English

Beak and feather disease virus (BFDV), belongs to the Circoviridae family and structurally has seven open reading frames (ORFs). Only two ORF C1 and ORF V1 are more affected by genetic changes. BFDV is prone to mutations that allow the circovirus to maintain its virulence. Because there are differences in replication rates between Rep, Cap, and intergenic sequences, a complete evaluation of the entire BFDV genome sequence is of great importance to determine all recombination opportunities. In this study, among the parrots referred to veterinary clinics in Isfahan (2021-2022), 300 parrots suspected of PBFD from the Cockatiel, Rose-ringed parakeet and African grey parrot species were selected for sampling. Approximately 4 to 8 feathers were removed from each bird showing signs of disease, and total DNA was isolated from clinical samples. Among the positive cases, 5 samples were selected and their nucleotide sequence was determined using the Sanger method on PCR products. Comparing the nucleotide sequence of the samples showed that these 5 viruses are divided into 3 branches. Nucleotide sequences from Cockatiel samples and African gray parrot samples were clustered with 97% similarity and with African gray parrot and Budgerigar samples isolated in Saudi Arabia in 2017. Isolated samples of Rose-ringed parakeet showed the highest nucleotide similarity to samples isolated from Rose-ringed parakeet and other parrots in Saudi Arabia and South Africa. In fact, the nucleotide sequence similarity of samples from Southeast Asia and Oceania is less than that of samples from Asia and Africa.

کلیدواژه‌ها English

PBFDV. Psittacine
Phylogeny
Mutation
Sequencing
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علوم درمانگاهی دامپزشکی ایران
دوره 18، شماره 1 - شماره پیاپی 30
بهار و تابستان 1403
مرداد 1403
صفحه 87-99